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h1n1 influenza a puerto rico 8 1934 virus  (ATCC)


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    ATCC h1n1 influenza a puerto rico 8 1934 virus
    H1n1 Influenza A Puerto Rico 8 1934 Virus, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 325 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/a+puerto+rico+8+1934/us12539279-776-4-9?v=ATCC
    Average 98 stars, based on 325 article reviews
    h1n1 influenza a puerto rico 8 1934 virus - by Bioz Stars, 2026-07
    98/100 stars

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    (A) Schematic of high-dose <t>H1N1</t> (LD 50 ) model over 21 days. (B) Survival curve of LysM -S1pr1 TG (n = 20) and control (n = 18) mice in response to H1N1 infection. Survival curves were compared using the Gehan–Breslow–Wilcoxon test. (C) Body weight loss of LysM -S1pr1 TG (n = 8) and control (n = 8) mice. (D) Blood oxygen saturation (SpO 2 ) in LysM -S1pr1 TG (n = 9) and control (n = 11) mice post-infection. Both (C) and (D) were tested by two-way mixed-effects analysis with Geisser–Greenhouse correction and Šídák’s multiple comparisons test; * p < 0.05, ** p < 0.01. (E) Schematic of the low-dose (0.16 LD 50 ) H1N1 model at 3 and 7 dpi. (F) Total cell count in BALF at 3 and 7 dpi (n = 7 mice per group). (G and H) BALF neutrophil and macrophage count at 3 and 7 dpi (n = 7 mice per group). (I-K) Expression of viral genes, including nucleoprotein (NP), polymerase acidic protein (PA), and hemagglutinin (HA), in the infected lung in control (n = 3) and LysM -S1pr1 TG (n = 4) mice. (L) Total protein in BALF in control (n = 3) and LysM -S1pr1 TG (n = 6) mice at 7 dpi. (M) IL-6 (ng/mL) secretion in BALF in control (n ≥ 4) and LysM- S1pr1 TG (n ≥ 5) mice at 3 and 7 dpi. (N) IL-10 (ng/mL) secretion in BALF in control (n ≥ 4) and LysM -S1pr1 TG (n ≥ 3) mice at 3 and 7 dpi. Data are mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 (two-tailed unpaired Student’s t test).
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    (A) Schematic of high-dose <t>H1N1</t> (LD 50 ) model over 21 days. (B) Survival curve of LysM -S1pr1 TG (n = 20) and control (n = 18) mice in response to H1N1 infection. Survival curves were compared using the Gehan–Breslow–Wilcoxon test. (C) Body weight loss of LysM -S1pr1 TG (n = 8) and control (n = 8) mice. (D) Blood oxygen saturation (SpO 2 ) in LysM -S1pr1 TG (n = 9) and control (n = 11) mice post-infection. Both (C) and (D) were tested by two-way mixed-effects analysis with Geisser–Greenhouse correction and Šídák’s multiple comparisons test; * p < 0.05, ** p < 0.01. (E) Schematic of the low-dose (0.16 LD 50 ) H1N1 model at 3 and 7 dpi. (F) Total cell count in BALF at 3 and 7 dpi (n = 7 mice per group). (G and H) BALF neutrophil and macrophage count at 3 and 7 dpi (n = 7 mice per group). (I-K) Expression of viral genes, including nucleoprotein (NP), polymerase acidic protein (PA), and hemagglutinin (HA), in the infected lung in control (n = 3) and LysM -S1pr1 TG (n = 4) mice. (L) Total protein in BALF in control (n = 3) and LysM -S1pr1 TG (n = 6) mice at 7 dpi. (M) IL-6 (ng/mL) secretion in BALF in control (n ≥ 4) and LysM- S1pr1 TG (n ≥ 5) mice at 3 and 7 dpi. (N) IL-10 (ng/mL) secretion in BALF in control (n ≥ 4) and LysM -S1pr1 TG (n ≥ 3) mice at 3 and 7 dpi. Data are mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 (two-tailed unpaired Student’s t test).
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    (A) Schematic of high-dose H1N1 (LD 50 ) model over 21 days. (B) Survival curve of LysM -S1pr1 TG (n = 20) and control (n = 18) mice in response to H1N1 infection. Survival curves were compared using the Gehan–Breslow–Wilcoxon test. (C) Body weight loss of LysM -S1pr1 TG (n = 8) and control (n = 8) mice. (D) Blood oxygen saturation (SpO 2 ) in LysM -S1pr1 TG (n = 9) and control (n = 11) mice post-infection. Both (C) and (D) were tested by two-way mixed-effects analysis with Geisser–Greenhouse correction and Šídák’s multiple comparisons test; * p < 0.05, ** p < 0.01. (E) Schematic of the low-dose (0.16 LD 50 ) H1N1 model at 3 and 7 dpi. (F) Total cell count in BALF at 3 and 7 dpi (n = 7 mice per group). (G and H) BALF neutrophil and macrophage count at 3 and 7 dpi (n = 7 mice per group). (I-K) Expression of viral genes, including nucleoprotein (NP), polymerase acidic protein (PA), and hemagglutinin (HA), in the infected lung in control (n = 3) and LysM -S1pr1 TG (n = 4) mice. (L) Total protein in BALF in control (n = 3) and LysM -S1pr1 TG (n = 6) mice at 7 dpi. (M) IL-6 (ng/mL) secretion in BALF in control (n ≥ 4) and LysM- S1pr1 TG (n ≥ 5) mice at 3 and 7 dpi. (N) IL-10 (ng/mL) secretion in BALF in control (n ≥ 4) and LysM -S1pr1 TG (n ≥ 3) mice at 3 and 7 dpi. Data are mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 (two-tailed unpaired Student’s t test).

    Journal: bioRxiv

    Article Title: S1PR1 signaling biases neutrophils toward long-lived low-inflammatory functional states

    doi: 10.64898/2026.02.05.703783

    Figure Lengend Snippet: (A) Schematic of high-dose H1N1 (LD 50 ) model over 21 days. (B) Survival curve of LysM -S1pr1 TG (n = 20) and control (n = 18) mice in response to H1N1 infection. Survival curves were compared using the Gehan–Breslow–Wilcoxon test. (C) Body weight loss of LysM -S1pr1 TG (n = 8) and control (n = 8) mice. (D) Blood oxygen saturation (SpO 2 ) in LysM -S1pr1 TG (n = 9) and control (n = 11) mice post-infection. Both (C) and (D) were tested by two-way mixed-effects analysis with Geisser–Greenhouse correction and Šídák’s multiple comparisons test; * p < 0.05, ** p < 0.01. (E) Schematic of the low-dose (0.16 LD 50 ) H1N1 model at 3 and 7 dpi. (F) Total cell count in BALF at 3 and 7 dpi (n = 7 mice per group). (G and H) BALF neutrophil and macrophage count at 3 and 7 dpi (n = 7 mice per group). (I-K) Expression of viral genes, including nucleoprotein (NP), polymerase acidic protein (PA), and hemagglutinin (HA), in the infected lung in control (n = 3) and LysM -S1pr1 TG (n = 4) mice. (L) Total protein in BALF in control (n = 3) and LysM -S1pr1 TG (n = 6) mice at 7 dpi. (M) IL-6 (ng/mL) secretion in BALF in control (n ≥ 4) and LysM- S1pr1 TG (n ≥ 5) mice at 3 and 7 dpi. (N) IL-10 (ng/mL) secretion in BALF in control (n ≥ 4) and LysM -S1pr1 TG (n ≥ 3) mice at 3 and 7 dpi. Data are mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 (two-tailed unpaired Student’s t test).

    Article Snippet: Influenza A/Puerto Rico 8/1934 (PR8) H1N1 strain was obtained from Charles River (Cat# 10100374, Wilmington, MA), aliquoted, and stored in liquid nitrogen.

    Techniques: Control, Infection, Cell Characterization, Expressing, Two Tailed Test

    (A) Kaplan–Meier survival curves of Mrp8- S1pr1 transgenic (TG) mice ( n = 25) and Cre-negative littermate controls ( n = 21) following intranasal infection with high-dose H1N1 (LD 50 ). Survival was monitored for up to 21 days post-infection. Statistical significance was assessed using the log-rank (Mantel–Cox) and Gehan–Breslow–Wilcoxon tests. (B) Body weight change in control (n = 13) and Mrp8- S1pr1 TG (n = 21) mice following influenza infection, expressed as a percentage of the initial body weight. Data represent mean ± SD. (C) Arterial oxygen saturation (SpO₂) measured longitudinally after infection using pulse oximetry in control (n = 13) and Mrp8- S1pr1 TG (n = 21) mice. Data represent mean ± SD. Statistical significance is denoted as * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: bioRxiv

    Article Title: S1PR1 signaling biases neutrophils toward long-lived low-inflammatory functional states

    doi: 10.64898/2026.02.05.703783

    Figure Lengend Snippet: (A) Kaplan–Meier survival curves of Mrp8- S1pr1 transgenic (TG) mice ( n = 25) and Cre-negative littermate controls ( n = 21) following intranasal infection with high-dose H1N1 (LD 50 ). Survival was monitored for up to 21 days post-infection. Statistical significance was assessed using the log-rank (Mantel–Cox) and Gehan–Breslow–Wilcoxon tests. (B) Body weight change in control (n = 13) and Mrp8- S1pr1 TG (n = 21) mice following influenza infection, expressed as a percentage of the initial body weight. Data represent mean ± SD. (C) Arterial oxygen saturation (SpO₂) measured longitudinally after infection using pulse oximetry in control (n = 13) and Mrp8- S1pr1 TG (n = 21) mice. Data represent mean ± SD. Statistical significance is denoted as * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: Influenza A/Puerto Rico 8/1934 (PR8) H1N1 strain was obtained from Charles River (Cat# 10100374, Wilmington, MA), aliquoted, and stored in liquid nitrogen.

    Techniques: Transgenic Assay, Infection, Control